Figure 5.

COX-2 gene expression in normal and HP fibroblasts exposed to pyridoxal phosphate (PLP) and/or calcium pyrophosphate (CPPD). Semiquantitative PCR was performed to analyze the expression of the COX 2 gene in normal and HP fibroblasts isolated from the skin and dura mater after exposure to PLP and/or CPPD in vitro. In order to allow comparisons, the expression was normalized to a 100% in each of the three groups of fibroblasts. Statistical analysis was done by 3-way ANOVA.